SEPARATION AND PURIFICATION OF PEPTIDES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

Preparative separations of peptides have been accomplished using high- speed counter-current chromatography. This has been made possible by t he use of a particular solvent system that does not exhibit solvent ca rryover at high speed flow and centrifugation conditions. The solvent system composed of tert.-butyl methyl ether, rt-butanol, acetonitrile and aqueous trifluoroacetic acid which can be adjusted in volume ratio s and percent acid is employed in two instruments for counter-current chromatography. The cross-axis coil planet centrifuge was used for sep aration of various dipeptides. Superior resolution was obtained with t he multi-layer coil planet centrifuge in the separation of six dipepti des and in the preparative purification of two synthetic peptides.