E. Gonzalez et al., DIRECT DETERMINATION OF DIURETIC DRUGS IN URINE BY CAPILLARY ZONE ELECTROPHORESIS USING FLUORESCENCE DETECTION, Journal of chromatography B. Biomedical applications, 687(1), 1996, pp. 145-150
Citations number
33
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
Four diuretic drugs banned in sport (amiloride, triamterene, bendroflu
methiazide and bumetanide) have been separated by capillary zone elect
rophoresis (CZE) and detected using conventional fluorescence spectrom
etry. The effect of pH on electrophoretic parameters such as migration
time, peak efficiency and peak height is discussed. Complete separati
on of the four drugs is achieved in less than 8 min at pH 8. No interf
erence due to endogenous urine components is observed and thus direct
urine analysis is feasible. Analytical figures of merit including prec
ision and limits of detection are presented. Limits of detection range
between 0.5 fmol for triamterene and 21.6 fmol for bumetanide.