DIRECT DETERMINATION OF DIURETIC DRUGS IN URINE BY CAPILLARY ZONE ELECTROPHORESIS USING FLUORESCENCE DETECTION

Four diuretic drugs banned in sport (amiloride, triamterene, bendroflu methiazide and bumetanide) have been separated by capillary zone elect rophoresis (CZE) and detected using conventional fluorescence spectrom etry. The effect of pH on electrophoretic parameters such as migration time, peak efficiency and peak height is discussed. Complete separati on of the four drugs is achieved in less than 8 min at pH 8. No interf erence due to endogenous urine components is observed and thus direct urine analysis is feasible. Analytical figures of merit including prec ision and limits of detection are presented. Limits of detection range between 0.5 fmol for triamterene and 21.6 fmol for bumetanide.