THE EFFECT OF THE 3'-TERMINAL MONOPHOSPHATE GROUP ON THE METAL-ION-PROMOTED HYDROLYSIS OF THE PHOSPHODIESTER BONDS OF SHORT OLIGONUCLEOTIDES

Authors
KUUSELA S AZHAYEV A GUZAEV A LONNBERG H
Citation
S. Kuusela et al., THE EFFECT OF THE 3'-TERMINAL MONOPHOSPHATE GROUP ON THE METAL-ION-PROMOTED HYDROLYSIS OF THE PHOSPHODIESTER BONDS OF SHORT OLIGONUCLEOTIDES, Perkin transactions. 2, (6), 1995, pp. 1197-1202
Citations number
30
Categorie Soggetti
Chemistry Physical","Chemistry Inorganic & Nuclear
Journal title
Perkin transactions. 2 → ACNP
ISSN journal
03009580
Issue
6
Year of publication
1995
Pages
1197 - 1202
Database
ISI
SICI code
0300-9580(1995):6<1197:TEOT3M>2.0.ZU;2-A
Abstract
The effect of 3'-terminal monophosphate group on the metal-ion-promote d hydrolysis of the phosphodiester bonds of oligonucleotides has been studied. For this purpose, the rate constants for the hydrolysis of th e following oligomers in the presence of Zn2+ and its 1,5,9-triazacycl ododecane chelate, Zn2+[12]aneN(3), have been determined: (i) ApUpUp(2 ') and ApUpUp(3'), (ii) Up(Tp)(n)T (n = 0-4 and 7), (iii) Up(Tp)(n)Tp (n = 0-4 and 7). The results obtained are used to propose a mechanism for the Zn2+ and Zn2+ [12]aneN(3) promoted hydrolysis of polynucleotid es.