SUPPRESSION OF CHIRAL RECOGNITION OF 3-HYDROXY-1,4-BENZODIAZEPINES DURING MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY WITH BILE-SALTS

During the development of a micellar electrokinetic chromatographic sc reening method for 1,4-benzodiazepines, peak splitting and broadening were observed for some 3-hydroxy-1,4-benzodiazepines (oxazepam, loraze pam, temazepam and lormetazepam). This phenomenon occurred when the mi cellar phase consisted of bile salts and can be ascribed to the chiral nature of these surfactants. As the bile salts were applied in order to reduce the capacity factors to an appropriate level, enantiomer sep aration was not an objective and even disturbing. By increasing the an alysis temperature, the chiral recognition of these compounds could be suppressed.