Yd. Hang et Ee. Woodams, BETA-FRUCTOFURANOSIDASE PRODUCTION BY ASPERGILLUS SPECIES FROM APPLE POMACE, Lebensmittel-Wissenschaft + Technologie, 28(3), 1995, pp. 340-342
Of the three Aspergillus species examined, A. foetidus NRRL 337 was fo
und to yield the highest beta-fructofurnnosidase activity after 96 h o
f cultivation at 30 degrees C on apple pomace. The enzyme was purified
about six-fold by ultrafiltration, gel filtration and DEAE-Sephadex A
-50 chromatography. Enzyme activity was optimal at 55 degrees C and pH
4.0. The enzyme had a K-m value of 260 mmol/L for sucrose and its mol
ecular weight was 200 kDa.