EVIDENCE FOR STIMULATION OF THE INOSITOL TRIPHOSPHATE-CA2-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI( SIGNALING SYSTEM IN RAT ENTEROCYTES BY HEAT)

Authors
CHAUDHURI AG GANGULY U
Citation
Ag. Chaudhuri et U. Ganguly, EVIDENCE FOR STIMULATION OF THE INOSITOL TRIPHOSPHATE-CA2-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI( SIGNALING SYSTEM IN RAT ENTEROCYTES BY HEAT), Biochimica et biophysica acta. Molecular cell research, 1267(2-3), 1995, pp. 131-133
Citations number
22
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Molecular cell research → ACNP
ISSN journal
01674889
Volume
1267
Issue
2-3
Year of publication
1995
Pages
131 - 133
Database
ISI
SICI code
0167-4889(1995)1267:2-3<131:EFSOTI>2.0.ZU;2-F
Abstract
In Escherichia coli heat stable enterotoxin (STa)-treated rat enterocy tes, the rise of inositol triphosphate (IP3) preceded the rise of [Ca2 +](i). Chelation of extracellular Ca2+ with EGTA and suspension of cel ls in Ca2+ free buffer both demonstrated the enterotoxin-induced initi al rise of [Ca2+](i) with a concomitant loss of sustained phase. Furth ermore, pretreatment of cells with dantrolene resulted in a decrease o f the early response of [Ca2+](i), indicating the initial effect of th e enterotoxin on the rise of [Ca2+](i) was mostly due to its mobilizat ion from some IP3-sensitive intracellular stores.