MOLECULAR-CLONING OF THE COMPLEMENTARY-DNA FOR THE MOUSE PYRUVATE-KINASE M-2 GENE WHOSE EXPRESSION IS DEPENDENT UPON CELL-DIFFERENTIATION

A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein contain ing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyru vate kinase. Expression of the pyruvate kinase in mouse embryonal carc inoma P19 cells altered according to differentiation stages; high at u ndifferentiated and low at differentiated stages.