DETERMINATION OF INDOLE ALKALOIDS FROM R-SERPENTINA AND R-VOMITORIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

The separation of a model mixture of six indole alkaloids by high-perf ormance liquid chromatography (HPLC) and high-performance thin-layer c hromatography (HPTLC) on the normal- and reversed-phase Armsorb suppor ts, respectively, was studied. A procedure for the analysis of extract s from plants cell callus culture of Rauwolfia serpentina and hairy ro ots of R. serpentina and R. vomitoria in the presence of these alkaloi ds and its derivatives with different chromatographic systems was elab orated. It was found that the alkaloid compositions of plant cell cult ures and hairy roots of R. serpentina and R. vomitoria are identical. With HPLC analysis a higher precision of alkaloid determination in sol ution and measurements of concentration is achieved. HPTLC analysis gi ves a qualitative identification of the extracts. The combination of t hese high-performance methods provides quantitative and qualitative an alyses of indole alkaloids such as ajmaline, ajmalicine, reserpine, ra ucaffricine, serpentine and yohimbine.