SEPARATION AND QUANTIFICATION OF TETRAETHYLENE GLYCOL MONOHEPTANOATE AND DIHEPTANOATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Tetraethylene glycol mono- and diheptanoate were prepared by direct es terification of heptanoic acid with tetraethylene glycol. The constitu ents of the reaction were separated by thin layer chromatography with flame ionization detection, but only tetraethyleneglycol diheptanoate could be determined by this method. The main constituents of the react ion mixture were quantified by HPLC using two different mobile phases on a non-bonded silica column. Acetonitrile acidified with 0.3% acetic acid was used to separate and quantify the monoester and tetraethylen e glycol, while hexane-isopropanol (80:20, v/v) acidified with 0.3% ac etic acid was used to separate and quantify heptanoic acid and the die ster. The starting compounds and the products of the reaction could be thus separated and quantified accurately using these two eluents with out derivatization of the hydroxylated compounds.