The efficacy of the ultrasonic standing plane wave in concentrating sm
all particles was theoretically evaluated and compared with experiment
al results. Acoustic energy density was estimated by measuring the ult
rasonic absorption, and particle distribution was observed by dark-fie
ld microscopy. The theory predicts that diffusion is negligible in con
centrating polystyrene spheres larger than 5 mu m in diameter when the
y are subjected to 2J/m(3) ultrasound. The half-width of the steady-st
ate particle distribution was of the same order of magnitude as the th
eoretical value for the particles of 1 mu m and 2 mu m diameter. We al
so applied this concentrating technique to fractionation of polystyren
e spheres 10 mu m in diameter, and more than 90% of the particles in t
he laminar flow were successfully collected.