ANALYSIS OF RECOMBINANT HUMAN GROWTH-HORMONE IN ESCHERICHIA-COLI FERMENTATION BROTH BY MICELLAR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method for the reversed-phase high-performance liquid chromatographi c (HPLC) determination of recombinant methionylaspartyl-human growth h ormone (MD-HGH) in Escherichia coli fermentation broth is described. T he technique utilizes mobile phases containing n-propanol and the anio nic surfactant sodium dodecyl sulfate (SDS) under micellar conditions at pH 6.4. The methodology is directly applicable to the analysis of s amples solubilized via sulfitolysis in the presence of SDS, and offers superior resolution in comparison with chromatography in the absence of the surfactant. Using this method, acceptable precision (day-to-day R.S.D. = 4.9%), accuracy, selectivity, range, linearity and ruggednes s were achieved.