BROADLY APPLICABLE POLYSILOXANE-BASED CHIRAL STATIONARY-PHASE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUPERCRITICAL-FLUID CHROMATOGRAPHY

A recently reported broad-spectrum chiral selector and two of its homo logs were incorporated into polymethylhydrosiloxane and each of the po lymers was immobilized on silica gel to afford chiral stationary phase s (CSPs) useful for the separation of the enantiomers of a wide variet y of compounds by HPLC and/or supercritical fluid chromatography (SFC) . These CSPs are stable toward normal-phase, reversed-phase, and sub/s upercritical fluid conditions and are not adversely affected by additi on of carboxylic acids or amines as mobile-phase modifiers. These poly meric CSPs show higher efficiencies and shorter analysis times when us ed with sub/supercritical carbon dioxide then when used in the HPLC mo de. Additionally, some separations are achievable using sub/supercriti cal carbon dioxide but not when the same column is used in the HPLC mo de.