F. Guardiola et al., ADSORPTION OF OXYSTEROLS ON DIFFERENT MICROTUBE MATERIALS DURING SILANYZATION PRIOR TO GAS-CHROMATOGRAPHIC DETERMINATION, Journal of chromatography, 705(2), 1995, pp. 396-399
The observation of increasing variability in the GC determination of o
xysterols when the material of the silanization microtubes was changed
led to a comparative study of three kinds of microtubes (Pyrex glass,
polypropylene homopolymer and polypropylene copolymer). The character
istics of the adsorption of each material on different oxysterols and
their influence on the reliability of the determination are reported.
The variability of the adsorption in the different materials is high f
or 5 alpha-cholestane, one of the most widely used internal standards
in the GC determination of oxysterols. This will affect the precision
and accuracy of the relative response factors. Significant differences
were also found for cholesterol and the five oxysterols analysed, esp
ecially between the polypropylene copolymer and the other two material
s.