A PHYSICAL MAP OF THE 85 KB VIRULENCE PLASMID OF RHODOCOCCUS-EQUI-103

A physical map of the 85 kb virulence plasmid pOTS from Rhodococcus eq ui 103 was constructed, The restriction map contains 2 AsnI, 5 BglII, 9 EcoRI, 4 HindHII, and 3 XbaI sites, The positions of the EcoRI and H indIII of pOTS are identical to that of the 85 kb virulence plasmid of R. equi ATCC 33701 reported recently by others. EcoRI restriction fra gment sizes were similar in the 85 kb plasmids isolated from 4 horse d erived R. equi but, except apparently for the 28.3 and possibly 2.0 an d 1.5 kb fragments, were different in an 80.1 kb plasmid isolated from a pig source R. equi.