Ah. Hussein et al., PREVALENCE OF SEROTYPES G6 AND G10 GROUP-A ROTAVIRUSES IN DAIRY CALVES IN QUEBEC, Canadian journal of veterinary research, 59(3), 1995, pp. 235-237
Fecal samples from diarrheic and nondiarrheic dairy calves (1 to 3 wee
ks old) from 12 regions of Quebec, collected between 1992 and 1994, we
re screened for group A bovine rotavirus (BRV) using a combination of
2 VP6-specific monoclonal antibodies (MAbs) in an enzyme-linked immuno
sorbent assay (ELISA). The overall prevalence of BRV infection was 26.
4% (107/405). In diarrheic calves, BRV infection reached 74.3% (55/74)
, but only 15.7% (52/331) in nondiarrheic calves. BRV-positive samples
were serotyped by enzyme-linked immunosorbent assay (ELISA) using G6
and G10 specific MAbs. The analysis of 107 field samples revealed that
, in diarrheic calves, 34.5% (19/55) were G6, 27.2% (15/55) were G10,
9% (5/55) were G6 and G10 positive, and 29.9% (16/55) were G6 and G10
negative. In nondiarrheic calves, 19.2% (10/52) were G6, 19.2% (10/52)
were G10, 7.6% (4/52) were G6 and G10 positive, and 53.6% (28/52) wer
e G6 and G10 negative. Rotavirus dsRNA was extracted from BRV-positive
samples and examined by polyacrilamide gel electrophoresis (PAGE). Of
107 samples tested, 74 (69.1%) were positive, and all the samples dem
onstrated a typical group A rotavirus migration pattern.