DETECTION OF ESCHERICHIA-COLI IN WATER USING A COLORIMETRIC GENE PROBE ASSAY

Authors
RICE EW COVERT TC JOHNSON SA JOHNSON CH REASONER DJ
Citation
Ew. Rice et al., DETECTION OF ESCHERICHIA-COLI IN WATER USING A COLORIMETRIC GENE PROBE ASSAY, Journal of environmental science and health. Part A: Environmental science and engineering, 30(5), 1995, pp. 1059-1067
Citations number
9
Categorie Soggetti
Environmental Sciences","Engineering, Environmental
Journal title
Journal of environmental science and health. Part A: Environmental science and engineering → ACNP
ISSN journal
10934529
Volume
30
Issue
5
Year of publication
1995
Pages
1059 - 1067
Database
ISI
SICI code
1093-4529(1995)30:5<1059:DOEIWU>2.0.ZU;2-P
Abstract
A commercially available DNA hydribization assay (Gene-trak(R), Framin gham, MA, USA) was compared with the EC-MUG procedure for the detectio n of Escherichia coil in water. The gene probe gave positive responses for pure cultures of E. coli, E. coli 0157:H7, E. fergusonii, Shigell a sonnei, S. dysenteriae and S. boydii. The hybridization assay was ca pable of detecting E. coil in environmental samples and survivors amon g chlorine exposed cells.