The axoneme of the spider spermatozoon (Pholcus sp.) has been examined
after fixation with a fixative that permits the visualization of the
individual protofilaments. The axoneme was found to consist of nine mi
crotubular doublers in a circle around three microtubular singlets, th
us a 9 + 3 pattern. Each of the three central microtubules contains 13
protofilaments and is flanked by two short filaments, maybe the equiv
alent of the central sheath. Distally the central microtubules are sli
ghtly flattened. The nine doublers have inner and outer dynein arms an
d have the same anatomy as have the doublers in regular 9 + 2 axonemes
. Whereas the nine doublers in 9 + 2 flagella have unequal positions i
n relation to the central microtubules and hence unequal tasks in flag
ellation, the same is nor true for the spider sperm axoneme.