BIOGENIC POLYAMINES AFFECT ACTIVITY OF AMINOPEPTIDASE-B AND ALANYL AMINOPEPTIDASE FROM PORCINE SKELETAL-MUSCLE

The effect of biogenic polyamines (agmatine, cadaverine,;putrescine, s permidine, and spermine) on the activity of pork muscle aminopeptidase and alanyl aminopeptidase was investigated. Agmatine (5 mM) showed a powerful inhibitory effect for both enzymes (more than 50% inhibition) . Cadaverine End less intensely putrescine showed similar inhibitory e ffects for both enzymes. All assayed polyamines inhibited activity of both aminopeptidases except spermine and spermidine which did not affe ct aminopeptidase B. Thus, one of these inhibitors could be used to av oid the interference of alanyl aminopeptidase when assaying aminopepti dase B in muscle extracts.