EFFECTS OF UVC IRRADIATION ON CULTURED MOUSE EMBRYONIC LIMB BUD CELLS

Effects of UVC irradiation (UVC) at a dose range from 0 to 30 J/m(2) w ere investigated on the cultured embryonic limb bud cells (LBC), isola ted from fore- and hindlimbs of day 11 mouse embryos. Although dose-de pendent inhibition was found for both cellular proliferation and chond rogenesis, the chondrogenic proteoglycan (PG) synthesis was more sensi tive to UVC than cellular proliferation when compared at ID50, the inh ibitory dose that reduced assessment value by 50% of the control. No s ignificant difference in induction and repair kinetics of UV-induced c yclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts ((6-4)PPs) were found between LBC and NIH3T3 mouse cell line. The fluorescent li ght (FL) treatment of LBC pre or post WC irradiation did not affect re pair kinetics of CPDs and (6-4)PPs, cellular proliferation, formation of PG-producing nodule and the PG synthesis.