KINETIC OF IN-VITRO GENERATION OF SOME HEMORPHINS - EARLY RELEASE OF LVV-HEMORPHIN-7, PRECURSOR OF VV-HEMORPHIN-7

Bovine globin has been hydrolysed by pepsin to different degrees of hy drolysis. Analysis of the hydrolysates, by reversed-phase high-perform ance liquid chromatography (RP-HPLC), shows the release of LW- and W-h emorphin-7. LW-hemorphin-7 was the first generated, at a degree of hyd rolysis (DH), as low as 4%. In contrast, W-hemorphin-7 was produced la ter. Our study clearly shows that W-hemorphin-7 is issued directly fro m LW-hemorphin-7, since this later completely disappeared during hydro lysis. This work allows us to suggest a possible pathway for in vivo h emorphins appearance.