DOPAMINE INHIBITS IN-VITRO RELEASE OF VIP AND PROLIFERATION OF VIP-IMMUNOREACTIVE PITUITARY-CELLS

Authors
CARRETERO J VAZQUEZ RJ SANTOS M CACICEDO L RUBIO M SANCHEZFRANCO F VAZQUEZ R
Citation
J. Carretero et al., DOPAMINE INHIBITS IN-VITRO RELEASE OF VIP AND PROLIFERATION OF VIP-IMMUNOREACTIVE PITUITARY-CELLS, Neuropeptides, 30(1), 1996, pp. 81-86
Citations number
42
Categorie Soggetti
Neurosciences,"Endocrynology & Metabolism
Journal title
Neuropeptides → ACNP
ISSN journal
01434179
Volume
30
Issue
1
Year of publication
1996
Pages
81 - 86
Database
ISI
SICI code
0143-4179(1996)30:1<81:DIIROV>2.0.ZU;2-H
Abstract
A double immunohistochemical study for VIP and proliferating cell nucl ear antigen (PCNA) was carried out on monolayer cultures from adult ma le rats pituitary glands treated with dopamine (ranging from 10(-9) to 10(-5) M), in order to establish whether or not dopamine is involved in the regulation of the proliferation rate of pituitary VIP-immunorea ctive cells. For all doses of dopamine assayed, the release of VIP to the culture medium, the numerical density of VIP-immunoreactive cells and the percentages of VIP- and PCNA-immunoreactive cells decreased si gnificantly after dopamine treatment. These results suggest that dopam ine could be a physiological inhibitor involved in the modulation of p ituitary VIP proliferation rate.