A POLYMORPHIC DINUCLEOTIDE REPEAT IN THE HUMAN DOPAMINE D5 RECEPTOR GENE PROMOTER

Previously, we have reported the cloning and characterization of the 5 '-flanking region of the human dopamine D5 receptor encoding gene (D5) and that the major transactivation domain was 119-182 bp upstream of the transcriptional start site [Beischlag, TV. et al., Biochemistry, 3 4 (1995) 5960-5970]. Within this region existed a small dinucleotide r epeat termed (TC)(13) In this report, we describe the screening of gen omic DNAs from 18 unrelated individuals by single-strand conformation polymorphism (SSCP) analysis. SSCP analysis revealed the existence of two additional alleles, termed (TC)(12) and (TC)(14). Neither form sig nificantly altered D5 promoter-mediated luciferase activity when compa red to that of the wild-type control, suggesting that small difference s in the number of dinucleotide repeats are not likely of any function al consequence for D5 transactivation.