INTERFERENCE BY GLICLAZIDE IN THE GLUCOSE OXIDASE PEROXIDASE METHOD FOR GLUCOSE ASSAY/

Gliclazide interferes with the glucose determination using the glucose oxidase/peroxidase (EC 1.1.3.4/1.11.1.7) (GOD-PERID) method utilizing 2',2-azino-di-(3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) as th e oxygen acceptor chromogen. There was an essentially linear relations hip between the concentrations of gliclazide and decreasing glucose re adings. One mu mol/l of gliclazide in samples leads to an apparent los s of about 2.5 mu mol/l of glucose. However, gliclazide did not interf ere with the glucose determination using the hexokinase/glucose-6-phos phate dehydrogenase method. This interference in the GOD-PERID method for glucose assay can occur in the in vitro experimental samples and c ause underestimation of the glucose values. It is suggested that caref ul attention should be paid to the limited applicability of the GOD-PE RID method for glucose assay.