BEADED REACTIVE POLYMERS .2. IMMOBILIZATION OF PENICILLIN-G ACYLASE ON GLYCIDYL METHACRYLATE DIVINYL BENZENE COPOLYMERS OF DIFFERING PORE-SIZE AND ITS DISTRIBUTION

Reactive, macroporous and beaded glycidyl methacrylate-divinyl benzene copolymers of controlled particle size were synthesised. The role of crosslink density, pore generating solvent type and its volume, concen trations of protective colloid and polymerisation initiator on the eff icacy of reacting with penicillin G acylase were investigated. The cov alent binding and expression of bound penicillin G acylase was found t o be altered by the different synthesis variables. Immobilised enzyme activity of 205 IU g(-1) was obtained with a polymer with 25 mol% cros slinking having a mean pore radius of 10.8 nm, generated with 43.5 cm( 3) of cyclohexanol as porogen.