Om. Memar et al., RECOMBINANT DESMOGLEIN-3 HAS THE NECESSARY EPITOPES TO ADSORB AND INDUCE BLISTER-CAUSING ANTIBODIES, Journal of investigative dermatology, 106(2), 1996, pp. 261-268
The development of an animal model for studying the pathogenesis of pe
mphigus vulgaris (PV) has been hampered by the unavailability of the p
urified full-length autoantigen desmoglein 3 (Dsg 3). Therefore, we ex
pressed Dsg 3 using a baculovirus expression system, The expressed pro
tein was identified as Dsg 3 by its reactivity with a pan-cadherin ant
i-serum, an anti-serum to a Dsg 3 synthetic peptide, or patient serum,
and by amino-terminal sequencing. Carbohydrate analysis showed that r
ecombinant Dsg 3 was glycosylated. While a majority of the recombinant
protein was cell associated, by immunoprecipitation, some Dsg 3 was d
emonstrated in the medium. The Dsg 3 could adsorb out blister-causing
antibodies from patient sera. Rabbit anti-Dsg 3 antibodies induced by
the recombinant Dsg 3 showed specific binding to intercellular spaces
of monkey esophagus by indirect immunofluorescence. Moreover, these an
tibodies induced PV-like blisters in neonatal mice and weakly bound pe
rilesional epidermis. Availability of large quantities of relatively p
ure Dsg 3 should now facilitate studies aimed at understanding Dsg 3 s
tructure and pathogenesis of PV, with implications for developing spec
ific immunotherapies.