MOLECULAR PROBES FOR SWEETENERS - IMMUNORECOGNITION OF SUPERPOTENT GUANIDINIUM COMPOUNDS

Monoclonal antibodies (MAb) were made to two different superpotent gua nidino sweet tasting ligands, p-cyanophenyl)-N'-(diphenylmethyl)-guani dineacetic acid and N-(p-cyanophenyl)-N'-(cyclooctyl)-guanidine-acetic acid, In the present study we examined the binding specificity of thr ee MAb clones (denoted as NC10.7, NC10.13, and NC37.3). The isotypes o f these MAb were determined to be IgG(1) kappa for NC10.7 and NC37.3, while NC10.13 was IgG(2b), kappa. The dissociation constants for the M Ab were 19 nM (NC10.7), 28 nM (NC10.13), and 16 nM (NC37.3). The bindi ng specificity of each MAb was characterized by a competitive inhibiti on radioimmunoassay using related sweetener analogues, Antibodies to t his family of sweet tasting compounds may be useful probes for the stu dy of sweet taste chemistry and identification of novel sweet taste li gands.