WHEN TO HARVEST PERIPHERAL-BLOOD STEM-CELLS AFTER MOBILIZATION THERAPY - PREDICTION OF CD34-POSITIVE CELL YIELD BY PRECEDING DAY CD34-POSITIVE CONCENTRATION IN PERIPHERAL-BLOOD

Purpose: To evaluate whether CD34(+) yield form a single peripheral-bl ood stem-cell (PBSC) harvest could To evaluate whether the CD34+ yield from predicted by measurement of the patient's circulating WBC and CD 34+ cell concentrations on the day before harvest. Patients and Method s: Thirty-nine patients with hematologic or nonhematologic malignancy underwent 41 stem-cell mobilization episodes with cytotoxic chemothera py and/or granulocyte colony-stimulating factor (G-CSF), and a total o f 63 leukapheresis procedures were performed. Peripheral-blood samples were analyzed for WBC and CD34+ cell concentrations both on the day b efore and the day of leukapheresis. Results: The median WBC and CD34() concentrations on the day preceding leukapheresis were 10.0 x 10(9)/ L (range, 0.4 to 44.4) and 24.9 x 10(6)/L (range, 0.1 to 349.4), respe ctively. On the day of harvest, the corresponding figures were 15.1 x 10(9)/L(range, 1.5 to 52.6) and 29.3 x 10(6)/L(range, 0.1 to 543.1), r espectively. The median CD34(+) cell number collected in a single leuk apheresis was 2.6 x 10(6)/kg body weight (range, 0.1 to 26.1). Both th e preceding day (r =.84, P <.001) and harvest day (r =.95, P <.001) CD 34(+) circulating concentrations correlated significantly with the num ber of CD34(+) cells per kilogram collected at leukapheresis. The corr elation between CD34(+) cells per kilogram collected and harvest day W BC count was also significant (r =.43, P <.001), but with the precedin g day WBC count was nonsignificant (r =.15, P >.2). Conclusion: The nu mber of CD34(+) cells harvested in a single leukapheresis can be predi cted by measurement of the preceding day peripheral-blood circulating CD34(+) concentration, and on the basis of these data a table of proba ble CD34+ cell yield has been constructed. This correlation may facili tate the efficient organization of leukapheresis procedures. (C) 1996 by American Society of Clinical Oncology.