A SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF BUCLIZINE HYDROCHLORIDE USING THE CHARGE-TRANSFER SPECTRUM OF BUCLIZINE IODINE COMPLEX

A simple and sensitive spectrophotometric method is described for the determination of buclizine hydrochloride in bulk and tablets form. The method is based on the formation Of charge-transfer complex between b uclizine,as n-donor, and iodine, as a acceptor, which measured spectro photometrically at 295 and 355 nm. A Job's plot indicated a 1:1 comple x between the drug and iodine and Beer's law was obeyed in a concentra tion range of 4-30 mu g ml(-1). A more detailed investigation of the c omplex was made with respect to its association constant and the free energy change. The method is simple and sensitive and has been applied successfully to the analysis of laboratory-made tablets without any i nterference from the tablet excipients. To validate the method, the re sults obtained were compared statistically with a newly developed UV-d erivative spectrophotometric method. The charge-tra nsf er method was favored due to its higher sensitivity, cheap coast and available equip ments.