H. Watanabe et al., TRANSIENT INCREASE OF BLOOD HISTAMINE LEVEL INDUCED BY PENTAGASTRIN -CONTINUOUS MONITORING BY IN-VIVO MICRODIALYSIS, Scandinavian journal of gastroenterology, 31(12), 1996, pp. 1144-1150
Background: Since few studies of (penta)gastrin-induced histamine rele
ase from the gastric mucosa into blood has been performed, an effect o
f pentagastrin on histamine level of rat blood was examined by using t
he in vivo microdialysis method. Methods: Pentagastrin was perfused th
rough the microdialysis probe implanted into the jugular vein of ureth
ane-anesthetized rats or in urethane-anesthetized, totally gastrectomi
zed rats, and dialysis samples of blood were concurrently collected. H
istidine decarboxylase (HDC) activities and histamine contents in the
glandular stomach and gastric acid output after pentagastrin stimulati
on were also investigated. Results: Pentagastrin induced a transient i
ncrease of blood histamine in a dose-dependent manner but failed to ca
use any increase of blood histamine in the totally gastrectomized rat.
Pentagastrin also induced increases of the HDC activity in the glandu
lar stomach and of the gastric acid output. The peak histamine level i
n blood occurred 40 min after pentagastrin perfusion, whereas the peak
acid secretion occurred after 80-120 min and then leveled off. Conclu
sions: The transient increase of blood histamine induced by pentagastr
in is attributable to the histamine released from enterochromaffin-lik
e cells and could be monitored by using the in vivo microdialysis meth
od.