TRANSIENT INCREASE OF BLOOD HISTAMINE LEVEL INDUCED BY PENTAGASTRIN -CONTINUOUS MONITORING BY IN-VIVO MICRODIALYSIS

Citation
H. Watanabe et al., TRANSIENT INCREASE OF BLOOD HISTAMINE LEVEL INDUCED BY PENTAGASTRIN -CONTINUOUS MONITORING BY IN-VIVO MICRODIALYSIS, Scandinavian journal of gastroenterology, 31(12), 1996, pp. 1144-1150
Citations number
36
Categorie Soggetti
Gastroenterology & Hepatology
ISSN journal
00365521
Volume
31
Issue
12
Year of publication
1996
Pages
1144 - 1150
Database
ISI
SICI code
0036-5521(1996)31:12<1144:TIOBHL>2.0.ZU;2-#
Abstract
Background: Since few studies of (penta)gastrin-induced histamine rele ase from the gastric mucosa into blood has been performed, an effect o f pentagastrin on histamine level of rat blood was examined by using t he in vivo microdialysis method. Methods: Pentagastrin was perfused th rough the microdialysis probe implanted into the jugular vein of ureth ane-anesthetized rats or in urethane-anesthetized, totally gastrectomi zed rats, and dialysis samples of blood were concurrently collected. H istidine decarboxylase (HDC) activities and histamine contents in the glandular stomach and gastric acid output after pentagastrin stimulati on were also investigated. Results: Pentagastrin induced a transient i ncrease of blood histamine in a dose-dependent manner but failed to ca use any increase of blood histamine in the totally gastrectomized rat. Pentagastrin also induced increases of the HDC activity in the glandu lar stomach and of the gastric acid output. The peak histamine level i n blood occurred 40 min after pentagastrin perfusion, whereas the peak acid secretion occurred after 80-120 min and then leveled off. Conclu sions: The transient increase of blood histamine induced by pentagastr in is attributable to the histamine released from enterochromaffin-lik e cells and could be monitored by using the in vivo microdialysis meth od.