ESTABLISHMENT AND CHARACTERIZATION OF HIGH-LUNG-METASTATIC AND LOW-LUNG-METASTATIC CELL-LINES DERIVED FROM MURINE COLON ADENOCARCINOMA-26 TUMOR LINE

We established and characterized high- (LuM1) and low-lung-metastatic (NM11) cell lines derived from murine colon adenocarcinoma 26 tumor li ne. LuM1 cell line was established as a clonal cell line from a cultur ed cell mixture derived from a lung-metastatic nodule after 7 sequenti al subcutaneous transplantations of lung-metastatic tumors in the abdo minal wall of BALB/c mice. NM11 cell line was established from a cultu red cell mixture derived from a subcutaneous transplant of murine colo n adenocarcinoma 26 tumor cells. LuM1 cells showed marked spontaneous lung metastases, but NM11 cells rarely did, High invasive potential of LuM1 cells was revealed by in vitro invasion assay using Matrigel rec onstituted membranes. Rapid retraction was observed in monolayers of h uman umbilical vein endothelial cells and bovine aortic endothelial ce lls when LuM1 cells were added an the monolayers. Gelatin zymography a nd immunochemical examinations with monoclonal antibodies against gela tinase B (M(r) 95,000 type IV collagenase) showed secretion of large a mounts of the gelatinase by LuM1 cells.