EFFECTS OF TEMPERATURE AND WHITE SUCKER (CATOSTOMUS-COMMERSONI) SERUMSUPPLEMENT ON THE IN-VITRO, MULTIPLICATION OF CRYPTOBIA-CATOSTOMI IN CELL-FREE CULTURE-MEDIUM
S. Li et Ptk. Woo, EFFECTS OF TEMPERATURE AND WHITE SUCKER (CATOSTOMUS-COMMERSONI) SERUMSUPPLEMENT ON THE IN-VITRO, MULTIPLICATION OF CRYPTOBIA-CATOSTOMI IN CELL-FREE CULTURE-MEDIUM, Parasitology research, 82(3), 1996, pp. 276-278
Cryptobia catostomi, a non-pathogenic haemoflagellate of white suckers
(Catostomus commersoni) multiplied rapidly in modified TDL-15 medium
supplemented with 10% fetal bovine serum and 1% heat-inactivated white
sucker serum (WSS) at 10 degrees C and 18 degrees C. The numbers of C
. catostomi counted were significantly higher in cultures incubated at
10 degrees C than in those incubated at 18 degrees C beginning at 3 w
eeks post-incubation. The culture forms (from the eighth subculture at
9 months after isolation) were morphologically similar to blood forms
and were infective to laboratory-raised white suckers. The parasite s
urvived for about 4 weeks in the medium without WSS at 18 degrees C. T
he present study indicates that WSS supplement supports the in vitro m
ultiplication of C. catostomi and that its multiplication is temperatu
re-dependent.