AN IMPROVED PROCEDURE FOR IN-SITU RTPCR

Authors
VOLPI L DONEDA L GRIMOLDI MG POLLINA E LARIZZA L
Citation
L. Volpi et al., AN IMPROVED PROCEDURE FOR IN-SITU RTPCR, Molecular and cellular probes, 10(1), 1996, pp. 63-66
Citations number
15
Categorie Soggetti
Cell Biology",Biology,"Biochemical Research Methods
Journal title
Molecular and cellular probes → ACNP
ISSN journal
08908508
Volume
10
Issue
1
Year of publication
1996
Pages
63 - 66
Database
ISI
SICI code
0890-8508(1996)10:1<63:AIPFIR>2.0.ZU;2-B
Abstract
Direct in situ RTPCR has been successfully applied to paraffin-embedde d human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by t he lack of any specific signal in parallel specimens where reverse tra nscription is omitted. (C) 1996 Academic Press Limited