CHARACTERISTICS OF TRANSGENIC TOBACCO CARRYING PHYTOHORMONE SYNTHESIST-DNA GENES

Tobacco cv. Samsun was transformed by two Agrobacterium tumefaciens bi nary vectors. The vector pCB1334 brought into the plant genome kanamyc in resistance marker and pTiC58 T-DNA gene 4. The pCB1349 vector also carried kanamycin resistance marker together with genes 1, 2 and 5. Th e re-generated transgenic plants showed several differences from untra nsformed controls on both physiological and morphological levels. All transgenic clones showed a decrease of dry matter content in compariso n with untransformed controls. Clones carrying transgene 4 showed a de creased level of chlorophylls, especially chlorophyll alpha.