M. Hayashi et al., INHIBITION OF MOUSE HEPATITIS-VIRUS MULTIPLICATION BY PHOSPHOROTHIOATE ANALOGS OF OLIGODEOXYNUCLEOTIDES COMPLEMENTARY TO THE LEADER RNA, Journal of veterinary medical science, 57(6), 1995, pp. 1081-1083
Phosphorothioate oligodeoxynucleotides (PS-oligo) complementary to a l
eader RNA of mouse hepatitis virus (MHV) were more effective inhibitor
s of MHV multiplication than natural oligodeoxynucleotides (PO-oligo).
Sequence-dependent inhibition of viral multiplication was shown at lo
w concentrations (0.001-0.1 mu M) of antisense PS-oligo. Phosphorothio
ate oligodeoxycytidine, PS-(dC)(20) and PS-oligo, which has no signifi
cant homology to the MHV sequence, showed inhibitory effects on MHV mu
ltiplication at concentrations higher than 0.5 mu M. These results sho
wed that PS-oligo was more potent than PO-oligo in inhibition of MHV m
ultiplication and that PS-oligo may inhibit MHV multiplication by two
different mechanisms, that is, in sequence-dependent and -independent
manners.