MOLECULAR-CLONING OF A CDNA-ENCODING ENOLASE FROM THE FILAMENTOUS FUNGUS, ASPERGILLUS-ORYZAE

Citation
M. Machida et al., MOLECULAR-CLONING OF A CDNA-ENCODING ENOLASE FROM THE FILAMENTOUS FUNGUS, ASPERGILLUS-ORYZAE, Current genetics, 30(5), 1996, pp. 423-431
Citations number
50
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
01728083
Volume
30
Issue
5
Year of publication
1996
Pages
423 - 431
Database
ISI
SICI code
0172-8083(1996)30:5<423:MOACEF>2.0.ZU;2-D
Abstract
A 1.6-kbp full-length cDNA for the Aspergillus oryzae enolase gene (en oA) was cloned. The sequenced insert contained a continuous open readi ng frame of 1314 bp encoding a protein of molecular weight 47 405. Amo ng all enolases sequenced to-date, the deduced aminoacid sequence show ed the highest homology (74.9%) with Candida albicans enolase (ENO1). Strong codon biases and multiple transcription start sites downstream from CT-blocks in the 5'-flanking region suggested strong expression. enoA mRNA was found to occupy approximately 3% (w/w) of total mRNA of A. oryzae by quantitative RT-PCR. This strong transcription was depend ent on the carbon source in the medium and correlated with the growth rate of the mycelium.