M. Machida et al., MOLECULAR-CLONING OF A CDNA-ENCODING ENOLASE FROM THE FILAMENTOUS FUNGUS, ASPERGILLUS-ORYZAE, Current genetics, 30(5), 1996, pp. 423-431
A 1.6-kbp full-length cDNA for the Aspergillus oryzae enolase gene (en
oA) was cloned. The sequenced insert contained a continuous open readi
ng frame of 1314 bp encoding a protein of molecular weight 47 405. Amo
ng all enolases sequenced to-date, the deduced aminoacid sequence show
ed the highest homology (74.9%) with Candida albicans enolase (ENO1).
Strong codon biases and multiple transcription start sites downstream
from CT-blocks in the 5'-flanking region suggested strong expression.
enoA mRNA was found to occupy approximately 3% (w/w) of total mRNA of
A. oryzae by quantitative RT-PCR. This strong transcription was depend
ent on the carbon source in the medium and correlated with the growth
rate of the mycelium.