A specially modified atomic-force microscope was used to measure the h
ardness of fully hydrated peritubular and intertubular dentine at two
locations within unerupted human third molars: within 1 mm of the dent
ine-enamel junction and within 1 mm of the pulp. The hardness of fully
hydrated peritubular dentine was independent of location, and ranged
from 2.23 to 2.54 GPa. The hardness of fully hydrated intertubular den
tine did depend upon location, and was significantly greater near the
dentine-enamel junction (values ranged from 0.49 to 0.52 GPa) than nea
r the pulp (0.12-0.18 GPa). A Nanoindenter was used to estimate the Yo
ung's modulus of dehydrated peritubular and intertubular dentine from
the unloading portion of the load displacement curve. The modulus valu
es averaged 29.8 GPa for the peritubular dentine (considered to be a l
ower limit), and ranged from 17.7 to 21.1 GPa for the intertubular den
tine, with the lower values obtained for dentine near the pulp.