G. Nicolotti et Gc. Varese, SCREENING OF ANTAGONISTIC FUNGI AGAINST AIR-BORNE INFECTION BY HETEROBASIDION-ANNOSUM ON NORWAY SPRUCE, Forest ecology and management, 88(3), 1996, pp. 249-257
In vitro inhibition of Heterobasidion annosum by fungi isolated from t
he surface and inside of Norway spruce (Picea abies L.) stumps, ten Ve
rticillium bulbillosum strains from Fagus sylvatica L. mycorrhizoplane
, and three lignivorous basidiomycetes from culture collections was ev
aluated at 5, 10 and 15 degrees C in a search for potential biocontrol
candidates. Forty-nine strains from nine genera and hyaline sterile m
ycelia with or without clamp connections were isolated from spruce. Ov
er 50% inhibition at one or more temperatures was displayed by 21 of 4
9 isolates. Fresh wood tests were also performed with the six fungi th
at displayed the highest in vitro activity and with the basidiomycetes
. The best results were observed with Hypholoma fasciculare, Trichoder
ma harzianum 7/AO, Verticillium bulbillosum (culture filtrate) and Pha
nerochaete velutina.