TRANSLOCATION OF P190RHO GUANOSINE TRIPHOSPHATASE-ACTIVATING PROTEIN FROM CYTOSOL TO THE MEMBRANE IN HUMAN NEUTROPHILS STIMULATED WITH DIFFERENT AGONISTS

In this paper, we investigated the subcellular distribution of p190rho guanosine triphosphatase-activating protein (p190 GAP) in human neutr ophils stimulated with different agonists. The results show that in ne utrophils treated with formyl-methionyl-leucyl-phenylalanine (FMLP) (1 ) p190 GAP was translocated from the cytosol to the membranes; (2) the translocation of p190 GAP took place only at doses of FMLP that induc ed the translocation of rac 1 and rac 2 and the activation of the NADP H oxidase; and (3) the kinetic of translocation of p190 GAP paralleled that of rac 1 and rac 2. However, when the agonist was concanavalin A (ConA) or phorbol 12-myristate 13-acetate (PMA), rac 1 and rac 2, but not the p190 GAP, were translocated. (C) 1996 Academic Press, Inc.