PHENOTYPIC AND FUNCTIONAL-CHARACTERIZATION OF CD8(-LYMPHOCYTES FROM THE CENTRAL-NERVOUS-SYSTEM OF RATS WITH CORONAVIRUS JHM INDUCED DEMYELINATING ENCEPHALOMYELITIS() T)

Intracerebral infection of Lewis (LEW) inbred rats with the neurotropi c strain of the murine coronavirus JHM (JHMV) frequently results in a monophasic paralytic disease. In contrast, infection of frown Norway ( BN) inbred rats does not lead to clinical disease. Previous findings i ndicated that in both rat strains brain-infiltrating leukocytes consis ted mainly of CD8(+) T lymphocytes. Here, we phenotypically as well as functionally characterised this T cell subset after isolation from th e central nervous system (CNS). Using JHMV-infected target cells, MHC class I restricted, cytotoxic T lymphocytes were demonstrated to be pr esent in the leukocyte fraction from the CNS of both, susceptible LEW and disease-resistant BN rats. However, compared to infected, but heal thy BN rats, diseased LEW rats generated an enhanced cytotoxic immune response which became most prominent at the maximum of neurological di sease. Recently published observations from our laboratory demonstrate d a strong virus-specific antibody response in the CNS of EN rats. In LEW rats, however, the response was delayed and of low magnitude. This suggests, that consequences of cytotoxic T lymphocyte action in JHMV- infected CNS tissue largely depend on the efficacy of an accompanying virus-specific humoral immune response.