B. Passlick et al., EXPRESSION OF MHC MOLECULES AND ICAM-1 ON NON-SMALL-CELL LUNG CARCINOMAS - ASSOCIATION WITH EARLY LYMPHATIC SPREAD OF TUMOR-CELLS, European journal of cancer, 32A(1), 1996, pp. 141-145
Early microdissemination of tumour cells determines the prognosis of p
atients with apparently localised non-small cell lung cancer (NSCLC).
Monoclonal antibodies to epithelial antigens can now be used to detect
single carcinoma cells present in mesenchymal secondary organs such a
s bone marrow or lymph nodes. The present study was designed to obtain
insights into the potential role of the immune system in lymphatic an
d haematogenous microdissemination of NSCLC cells. Using immunohistoch
emical staining of primary NSCLC, we assessed the expression pattern o
f molecules mediating an efficient cellular immune response, that is,
MHC class I and class II antigens and the intercellular adhesion molec
ule-1 (ICAM-1). All 58 patients evaluated were staged as free of overt
metastases by conventional clinico-pathological screening. Isolated t
umour cells in bone marrow or lymph nodes were identified with mAb CK2
to cytokeratin component No. 18 and mAb BerEp-4 to glycoproteins of 3
4 and 39 kd present on epithelial cells, respectively. MHC class I exp
ression on primary tumours was reduced or absent in 6/10 (60.0%) patie
nts with isolated cancer cells in lymph nodes as compared to 6/33 tumo
urs (18.1%) without such tumour cell dissemination (P = 0.01). MHC cla
ss II molecules on primary tumours were detected in 1/10 (10.0%) patie
nts with micrometastases to regional lymph nodes and in 10/33 (30.3%)
patients without such a tumour cell spread. None of the 10 patients wi
th nodal microdissemination expressed ICAM-1 on their primary NSCLC, w
hile such expression was detectable in 12/33 (36.4%) patients without
this dissemination (P = 0.01). In contrast, the detection of tumour ce
lls in bone marrow was not correlated to the expression of any of thes
e immunoregulatory molecules. Our data suggest that escape caused by d
eficient expression of MHC class I antigens and ICAM-1 on tumour cells
may support homing or survival of disseminated tumour cells in lympho
id tissue.