IDENTIFICATION AND CHARACTERIZATION OF 3 GENES AND 2 PSEUDOGENES ON CHROMOSOME-13

A study was conducted on the feasibility of isolating genes and pseudo genes that map to chromosome 13 by a hybridization-based approach usin g a 13-specific library and pools of repeat-free cDNA clones. Five pai rs of cDNA and chromosome 13 genomic clones were identified and charac terized. Partial or full-length sequence was derived from all cDNAs, a nd database searches were performed for putative gene identification, Partial sequence was also obtained from the chromosome 13 genomic clon es for comparison with those of the hybridizing cDNAs. As a result of these analyses we identified three genes, a putative homologue of a po rcine mRNA encoding an unidentified hepatic protein, a putative homolo gue of a yeast integral membrane protein, and a gene for a translation ally controlled tumor protein, and two processed pseudogenes, ribosoma l proteins L23a and S3a. The latter was formerly identified as the v-f os transformation effector gene, Fte-1, and recently cited as a possib le candidate for the BRCA2 gene on chromosome 13. All genes and pseudo genes were localized to cytogenetic bands by in situ hybridization of metaphase chromosomes with probes derived from the chromosome 13 genom ic clones.