The Y chromosome gene SRY plays an important role in normal male sexua
l development and is thought to be the testis-determining factor. We d
escribe a familial nonsense mutation in SRY, shared by two XY sisters
with complete gonadal dysgenesis and, in a mosaic manner, by their fat
her. This mutation, consisting of a C to T transition in position 1 of
codon 97 of SRY,results in a truncated peptide with an incomplete DNA
-binding domain. The mutation is also present in the father of the two
cases, but a portion of wild-type SRY also remains. Our data suggest
that the father suffered a postzygotic mutation early in development,
but that he retained a remnant of functional SRY protein that accounts
for his normal development.