AN INTEGRATED MAP WITH COSMID PAC CONTIGS OF A 4-MB DOWN-SYNDROME CRITICAL REGION/

The major phenotypic features of Down syndrome have been correlated wi th partial trisomies of chromosome 21, allowing us to define the candi date gene region to a 4-Mb segment on the 21q22.2 band, We present her e a high-resolution physical map with megabase-sized cosmid/PAC contig s, This ordered clone library has provided unique material for the int egration of a variety of mappable objects, including exons, cDNAs, res triction sites, etc. Furthermore, our results have exemplified a strat egy for the completion of the chromosome 21 map to sequencing. (C) 199 6 Academic Press, Inc.