WALKING, CLONING, AND MAPPING WITH YACS IN 3Q27 - LOCALIZATION OF 5 ESTS INCLUDING 3 MEMBERS OF THE CYSTATIN GENE FAMILY AND IDENTIFICATIONOF CPG ISLANDS

Using yeast artificial chromosomes, we have generated a high-resolutio n physical map for 2.7 Mb of human chromosomal region 3q27. The YAC cl ones group into three contigs, one of which has also been linked to th e CFPH YAC contig map of human chromosome 3. Fluorescence in situ hybr idization has been used to order the contigs on the chromosome and to estimate the distance between them. Expressed sequence tags for five g enes, including three members of the cystatin gene family and a gene t hought to be involved in B-cell non-Hodgkin lymphoma, have been placed within the YAC contigs, and 12 putative CpG islands have been identif ied. These YACs provide a useful resource to complete the physical map ping of 3q27 and to bean identification and characterization of furthe r genes that are located there. (C) 1996 Academic Press, Inc.