THE GENE FOR HUMAN GLUTAREDOXIN (GLRX) IS LOCALIZED TO HUMAN-CHROMOSOME 5Q14

Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-depend ent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH, and glutathione reductase. A cDNA encoding the human glutaredoxi n gene (HGMW-approved symbol GLRX) has recently been isolated and clon ed from a human fetal spleen cDNA library. The screening of a human ge nomic library in Charon 4A led to the identification of three genomic clones. Using fluorescence in situ hybridization to metaphase chromoso mes with one genomic clone as a probe, the human glutaredoxin gene was localized to chromosomal region 5q14. This localization at chromosome 5 was in agreement with the somatic cell hybrid analysis, using DNA f rom a human-hamster and a human-mouse hybrid panel and using a human g lutaredoxin cDNA as a probe. (C) 1996 Academic Press, Inc.