Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-depend
ent disulfide oxidoreduction reactions in a coupled system with NADPH,
GSH, and glutathione reductase. A cDNA encoding the human glutaredoxi
n gene (HGMW-approved symbol GLRX) has recently been isolated and clon
ed from a human fetal spleen cDNA library. The screening of a human ge
nomic library in Charon 4A led to the identification of three genomic
clones. Using fluorescence in situ hybridization to metaphase chromoso
mes with one genomic clone as a probe, the human glutaredoxin gene was
localized to chromosomal region 5q14. This localization at chromosome
5 was in agreement with the somatic cell hybrid analysis, using DNA f
rom a human-hamster and a human-mouse hybrid panel and using a human g
lutaredoxin cDNA as a probe. (C) 1996 Academic Press, Inc.