PHYSICAL MAPPING OF 49 MICROSATELLITE MARKERS ON CHROMOSOME-19 AND CORRELATION WITH THE GENETIC-LINKAGE MAP

We have regionally localized 49 microsatellite markers developed by Ge nethon using a panel of previously characterized somatic cell hybrids that retain fragments from chromosome 19. The tight correlation observ ed between the physical and the genetic orders of the microsatellites provide cytogenetic anchorages to the genetic map data. We propose a p osition for the centromere just above D19S415, from the study of two h ybrids, each of which retains one of the two derivatives of a balanced translocation t(1;19)(q11;q11). Microsatellites, which can be identif ied by a standard PCR protocol, are useful tools for the localization of disease genes and for the establishment of YAC or cosmid contigs. T hese markers can also judiciously be used for the characterization of new hybrid cell line panels. We report such a characterization of 11 c lones, 8 of which were obtained by irradiation-fusion. Using the whole hybrid panel, we were able to define the order of 12 pairs of genetic ally colocalized microsatellites. As examples of gene mapping by the c ombined use of microsatellites and hybrid cell lines, we regionally as signed the PVS locus between the 19q13.2 markers D19S417 and D19S423 a nd confirmed the locations of fucosyltransferase loci FUT1, FUT2, and FUT5. (C) 1996 Academic Press, Inc.