A NOVEL HUMAN GENE ENCODING A C-PROTEIN-COUPLED RECEPTOR (GPR15) IS LOCATED ON CHROMOSOME-3

We used sequence similarities among G-protein-coupled receptor genes t o discover a novel receptor gene. Using primers based on conserved reg ions of the opioid-related receptors, we isolated a PCR product that w as used to locate the full-length coding region of a novel human recep tor gene, which we have named GPR15. A comparison of the amino acid se quence of the receptor encoded by GPR15 with other receptors revealed that it shared sequence identity with the angiotensin II AT1 and AT2 r eceptors, the interleukin 8b receptor, and the orphan receptors GPR1 a nd AGTL1. GPR15 was mapped to human chromosome 3q11.2-q13.1. (C) 1996 Academic Press, Inc.