We investigate why vessels that contain blood, which has a red or a da
rk, red color, may look bluish in human tissue. A CCD camera was used
to make images of diffusely reflected light at different wavelengths.
Measurements of reflectance that are due to model blood vessels in sca
ttering media and of human skin containing a prominent vein are presen
ted. Monte Carlo simulations were used to calculate the spatially reso
lved diffuse reflectance for both situations. We show that the color o
f blood vessels is determined by the following factors: (i) the scatte
ring and absorption characteristics of skin at different wavelengths,
(ii) the oxygenation state of blood, which affects its absorption prop
erties, (iii) the diameter and the depth of the vessels, and (iv) the
visual perception process. (C) 1996 Optical Society of America