EXPOSURE TO URBAN AND RURAL AIR-POLLUTION - DNA AND PROTEIN ADDUCTS AND EFFECT OF GLUTATHIONE-S-TRANSFERASE GENOTYPE ON ADDUCT LEVELS

Ambient air in urban areas is polluted by agents suspected of causing cancer in humans. A number of epidemiological studies have revealed an increased cancer risk in urban communities, especially in lung cancer . The relative risk have been estimated to be in the order of 1.5. The objective of this study was to evaluate differences in genotoxic expo sure through air pollution in urban and rural areas using DNA and prot ein adducts as biomarkers. Another objective was to investigate whethe r the GSTM1 genotype has any effect on adduct level. The analyses incl uded P-32 postlabelling of DNA adducts in lymphocytes, enzyme-linked i mmunosorbent assay for measuring benzo[a]pyrene protein adducts and po lymerase chain reaction amplification of the GSTM1 genotype. The study was a cross-sectional study of non-smoking, healthy males from rural and urban Danish areas and from Athens, Greece. All individuals in the study were healthy, non-smoking males. The Danish urban group include d 74 university students, the rural group 29 students from agricultura l colleges and the Greek group 17 individuals. Adduct levels differed significantly in the three groups with median levels of 0.152 fmol/mu g DNA (rural), 0.205 fmol/mu g (urban) and 0.285 fmol/mu g (Athens). T he adduct patterns showed some identical spots, but also specific addu cts. Here we report increasing DNA adduct levels comparing residents i n rural, small urban and large urban residential areas; we found no in fluence of GSTM1 genotype on DNA or protein adduct levels in non-smoke rs exposed to low levels of air pollution.