ISOLATION AND CHARACTERIZATION OF A PSEUDOMONAS-AERUGINOSA DNA-SEQUENCE, ENCODING A PUTATIVE DNA HELICASE BELONGING TO SUPERFAMILY-I

The isolation and characterization of a 2,122 bp DNA fragment from a P seudomonas aeruginosa genomic library containing a 1,495 bp open readi ng frame (ORF) encoding a putative DNA helicase is described. The dedu ced amino acid (498 residues) sequence derived from this ORF, exhibite d a high degree of homology to the rep and uvrD helicases of Escherich ia coli (55 and 38% identities, respectively), and to those of Staphyl ococcus aureus and Haemophilus influenzae (43 and 39% identities, resp ectively), all of them belonging to superfamily I.